1,997 research outputs found
Prevalence of refractive error in Bangladeshi adults - Results of the national blindness and low vision survey of Bangladesh
Get PDFMüller glia activation in response to inherited retinal degeneration is highly varied and disease-specific
Get PDFDespite different aetiologies, most inherited retinal disorders culminate in photoreceptor loss, which induces concomitant changes in the neural retina, one of the most striking being reactive gliosis by Müller cells. It is typically assumed that photoreceptor loss leads to an upregulation of glial fibrilliary acidic protein (Gfap) and other intermediate filament proteins, together with other gliosis-related changes, including loss of integrity of the outer limiting membrane (OLM) and deposition of proteoglycans. However, this is based on a mix of both injury-induced and genetic causes of photoreceptor loss. There are very few longitudinal studies of gliosis in the retina and none comparing these changes across models over time. Here, we present a comprehensive spatiotemporal assessment of features of gliosis in the degenerating murine retina that involves Müller glia. Specifically, we assessed Gfap, vimentin and chondroitin sulphate proteoglycan (CSPG) levels and outer limiting membrane (OLM) integrity over time in four murine models of inherited photoreceptor degeneration that encompass a range of disease severities (Crb1rd8/rd8, Prph2+/Δ307, Rho-/-, Pde6brd1/rd1). These features underwent very different changes, depending upon the disease-causing mutation, and that these changes are not correlated with disease severity. Intermediate filament expression did indeed increase with disease progression in Crb1rd8/rd8 and Prph2+/Δ307, but decreased in the Prph2+/Δ307 and Pde6brd1/rd1 models. CSPG deposition usually, but not always, followed the trends in intermediate filament expression. The OLM adherens junctions underwent significant remodelling in all models, but with differences in the composition of the resulting junctions; in Rho-/- mice, the adherens junctions maintained the typical rod-Müller glia interactions, while in the Pde6brd1/rd1 model they formed predominantly between Müller cells in late stage of degeneration. Together, these results show that gliosis and its associated processes are variable and disease-dependent
Impact of BREXIT on UK Gene and Cell Therapy: The Need for Continued Pan-European Collaboration
Get PDFCONTESTATION BEHIND TOLERANCE: BETWEEN COMPETITION AND TOLERANCE IN THE DISCOURSE OF MULTICULTURALISM IN SALATIGA
Get PDFTolerance must be close to pluralism and multiculturalism which is not a new thing in academic studies. Many studies and research generalize about respect and appreciation for the diversity of ethnicity, religion, language and other cultures. For Javanese people who tend to be homogeneous in ethnicity but not too many long conflicts arise. Truth, the proof, One of the results of research in Salatiga which is one of the student cities in Central Java received the title of the second most tolerant city in Indonesia in 2018. But behind the award, there was contestation in the fields of education and religion. This paper will look again at tolerance in the Salatiga community after the award was obtained. Besides that, it also discussed competition in academia at IAIN Salatiga and Satya Wacana Kristen University (UKSW). Both educational institutions are based on religion, in which multiculturalism. Both educational institutions are based on religion, which is multicultural so that it impacts on the contestation of institutional policies, facilities and theological expansion. The results of this study are obtained from social phenomena which are understood by the phenomenology paradigm from reality understood in consciousness. In addition, during activities in Salatiga, there were also some social realities about tolerance and competition in the field of education. Finally, tolerance that has gone well does not mean without contestation. Contestation in this case is one of the social and cultural dynamics. Even to achieve social integration, sometimes conflict with good management is needed. This means that contestation or conflict does not always lead to division, but rather one of the paths to integration
Between the Elite Power and Women Subordination: Formulation of "Dana Desa" in Java Village Context
Get PDFDana Desa is one of the new policies to realize development based on the needs of rural communities. In its implementation there is a role that is often overlooked, it is women. This paper discusses the process of formulating dana desa policies in Pengalusan Village. In addition, the main focus is on the role of women when formulating these policies. Applying gender approach this study found that the planning process for the formulation of dana desa policies is carried out with the musrenbangdes which is carried out before the APBDes decision is agreed. But from the planning through evaluation processes there is no specific discussion related to the ideas, interest, and the role of women in the development processes in the village. The elite as decision makers are men, and village development policies are more masculine, one of which is demonstrated through physical development. Women's ideas have not received enough attention
Impression Management Komunikasi Lintas Agama Di Sorong, Papua Barat
Get PDFIndonesia as a country with a diversity of religions provides part of a strong guideline in the society. The purpose of this study is to describe the management of impressions in inter-religious communication in Sorong, West Papua. The approach of research uses a phenomenology of descriptive-qualitative type with the perspective of dramaturgy theory. Moreover, the data were collected through interviews with religious leaders of Papua, while the observations were made to understand the situation of social interaction in society as well as documentation related to research themes. Furthermore, the data analysis is by grouping information, making categories, and producing findings. Meanwhile, the results of this study will describe that the process of managing impressions in interreligious communication in the Sorong, West Papua is the appearance of stage impressions, appearances, and behavioral styles. Stage impression management is done externally and internally. In addition, the external stage performance was carried out through the Religious Communication Forum (FKUB/Forum Komunikasi Umat Beragama) meeting, while the internal stage was held in a meeting at a place of worship. As for the appearance and style of behavior based on local Papuan customs by the traditional guidelines and religious teachings adopted. Therefore, the overall management of impressions by religious communities as a social capital that could minimize horizontal conflict in the name of religio
Gene therapy restores vision in rd1 mice after removal of a confounding mutation in Gpr179
Get PDFThe rd1 mouse with a mutation in the Pde6b gene was the first strain of mice identified with a retinal degeneration. However, AAV-mediated gene supplementation of rd1 mice only results in structural preservation of photoreceptors, and restoration of the photoreceptor-mediated a-wave, but not in restoration of the bipolar cell-mediated b-wave. Here we show that a mutation in Gpr179 prevents the full restoration of vision in rd1 mice. Backcrossing rd1 with C57BL6 mice reveals the complete lack of b-wave in a subset of mice, consistent with an autosomal recessive Mendelian inheritance pattern. We identify a mutation in the Gpr179 gene, which encodes for a G-protein coupled receptor localized to the dendrites of ON-bipolar cells. Gene replacement in rd1 mice that are devoid of the mutation in Gpr179 successfully restores the function of both photoreceptors and bipolar cells, which is maintained for up to 13 months. Our discovery may explain the failure of previous gene therapy attempts in rd1 mice, and we propose that Grp179 mutation status should be taken into account in future studies involving rd1 mice
Harnessing the Potential of Human Pluripotent Stem Cells and Gene Editing for the Treatment of Retinal Degeneration
Get PDFPURPOSE OF REVIEW: A major cause of visual disorders is dysfunction and/or loss of the light-sensitive cells of the retina, the photoreceptors. To develop better treatments for patients, we need to understand how inherited retinal disease mutations result in the dysfunction of photoreceptors. New advances in the field of stem cell and gene editing research offer novel ways to model retinal dystrophies in vitro and present opportunities to translate basic biological insights into therapies. This brief review will discuss some of the issues that should be taken into account when carrying out disease modelling and gene editing of retinal cells. We will discuss (i) the use of human induced pluripotent stem cells (iPSCs) for disease modelling and cell therapy; (ii) the importance of using isogenic iPSC lines as controls; (iii) CRISPR/Cas9 gene editing of iPSCs; and (iv) in vivo gene editing using AAV vectors. RECENT FINDINGS: Ground-breaking advances in differentiation of iPSCs into retinal organoids and methods to derive mature light sensitive photoreceptors from iPSCs. Furthermore, single AAV systems for in vivo gene editing have been developed which makes retinal in vivo gene editing therapy a real prospect. SUMMARY: Genome editing is becoming a valuable tool for disease modelling and in vivo gene editing in the retina
Pengetahuan, sikap Remaja Putri dan Dukungan Petugas Kesehatan terhadap Konsumsi Tablet Besi Folat SMKN 1 Bangsri Jepara: Sebuah Studi Cross Sectional
Get PDFThe iron-folate tablets program on adolescent girls for anemia prevention still facing some obstacles, especially adherence to consuming them. This study aimed to determine the relationship between knowledge, attitudes, and health worker’s support with the adherence of iron-folate tablets consumption. This study was a cross-sectional study, conducted at SMKN 1 Bangsri Jepara. Anumber of 73 respondents were selected by stratified random sampling. The data were obtained through interviews using a questionnaire, and analyzed using Rank Spearman test.The results showed that most respondents had moderate knowledge (54.8%), good attitude (69.9%), and good health worker’s support (65.8%). As much as 82.2% of adolescent girls did not comply to consume iron-folate tablet which were given once in a week. The unpleasant smell and taste of iron-folate was the reason for the respondent’s (31,5%) disiobedient. The statistical tests showed that there were relationships between knowledge and respondent’s compliance (p=0.004;r=0.334), attitude and respondent’s compliance (p=0.000;r=0.543) and health worker’s support with the respondent’s compliance (p=0.000;r=0.544).The health worker’s support variable is the most dominant variable related to the compliance of iron-folate tablets consumption. It is shown that the higher the support from health workers, the higher the compliance of iron-folate tablets consumption.Keywords: Compliance, adolescent girl, iron-folate tablet consumptio
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