CORE

🇺🇦   make metadata, not war

    94 research outputs found

    Female Behaviour Drives Expression and Evolution of Gustatory Receptors in Butterflies

    Author
    Publication venue
    'Public Library of Science (PLoS)'
    Publication date
    Field of study
    Get PDF
    Secondary plant compounds are strong deterrents of insect oviposition and feeding, but may also be attractants for specialist herbivores. These insect-plant interactions are mediated by insect gustatory receptors (Grs) and olfactory receptors (Ors). An analysis of the reference genome of the butterfly Heliconius melpomene, which feeds on passion-flower vines (Passiflora spp.), together with whole-genome sequencing within the species and across the Heliconius phylogeny has permitted an unprecedented opportunity to study the patterns of gene duplication and copy-number variation (CNV) among these key sensory genes. We report in silico gene predictions of 73 Gr genes in the H. melpomene reference genome, including putative CO2, sugar, sugar alcohol, fructose, and bitter receptors. The majority of these Grs are the result of gene duplications since Heliconius shared a common ancestor with the monarch butterfly or the silkmoth. Among Grs but not Ors, CNVs are more common within species in those gene lineages that have also duplicated over this evolutionary time-scale, suggesting ongoing rapid gene family evolution. Deep sequencing (∼1 billion reads) of transcriptomes from proboscis and labial palps, antennae, and legs of adult H. melpomene males and females indicates that 67 of the predicted 73 Gr genes and 67 of the 70 predicted Or genes are expressed in these three tissues. Intriguingly, we find that one-third of all Grs show female-biased gene expression (n = 26) and nearly all of these (n = 21) are Heliconius-specific Grs. In fact, a significant excess of Grs that are expressed in female legs but not male legs are the result of recent gene duplication. This difference in Gr gene expression diversity between the sexes is accompanied by a striking sexual dimorphism in the abundance of gustatory sensilla on the forelegs of H. melpomene, suggesting that female oviposition behaviour drives the evolution of new gustatory receptors in butterfly genomes
    Crossref
    Harvard University - DASH
    Directory of Open Access Journals
    HAL Descartes
    PubMed Central
    UCL Discovery
    eScholarship - University of California
    ProdInra
    HAL: Hyper Article en Ligne
    The Francis Crick Institute

    Finishing the euchromatic sequence of the human genome

    Author
    Publication venue
    LSU Digital Commons
    Publication date
    Field of study
    Get PDF
    The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead
    LSU Scholarly Repository (Louisiana State Univ.)

    Abstracts from the 20th International Symposium on Signal Transduction at the Blood-Brain Barriers

    Author
    Publication venue
    'Springer Science and Business Media LLC'
    Publication date
    Field of study
    Full text link
    https://deepblue.lib.umich.edu/bitstream/2027.42/138963/1/12987_2017_Article_71.pd
    Crossref
    Directory of Open Access Journals
    University of Miami: Scholarship Miami
    Deep Blue Documents

    Discutindo a educação ambiental no cotidiano escolar: desenvolvimento de projetos na escola formação inicial e continuada de professores

    Author
    Publication venue
    Publication date
    Field of study
    Get PDF
    A presente pesquisa buscou discutir como a Educação Ambiental (EA) vem sendo trabalhada, no Ensino Fundamental e como os docentes desta escola compreendem e vem inserindo a EA no cotidiano escolar., em uma escola estadual do município de Tangará da Serra/MT, Brasil. Para tanto, realizou-se entrevistas com os professores que fazem parte de um projeto interdisciplinar de EA na escola pesquisada. Verificou-se que o projeto da escola não vem conseguindo alcançar os objetivos propostos por: desconhecimento do mesmo, pelos professores; formação deficiente dos professores, não entendimento da EA como processo de ensino-aprendizagem, falta de recursos didáticos, planejamento inadequado das atividades. A partir dessa constatação, procurou-se debater a impossibilidade de tratar do tema fora do trabalho interdisciplinar, bem como, e principalmente, a importância de um estudo mais aprofundado de EA, vinculando teoria e prática, tanto na formação docente, como em projetos escolares, a fim de fugir do tradicional vínculo “EA e ecologia, lixo e horta”.Facultad de Humanidades y Ciencias de la Educació
    Publikationsserver der RWTH Aachen University
    Servicio de Difusión de la Creación Intelectual

    Listen carefully

    Author
    Publication venue
    Mark Allen Group
    Publication date
    Field of study
    Full text link
    The art of listening has an important role to play in raising the quality of classroom dialogue and the learning that goes with it. Jamie Thom offers strategies to ensure that all children – whether they are naturally quiet or extrovert – derive maximum benefit. </jats:p
    Crossref

    The Essentials in Learning to Teach: Thriving as a new secondary teacher

    Author
    Publication venue
    Publication date
    Field of study
    No full text
    Repository@Napier

    A sequential therapy with sorafenib followed by regorafenib against single-line atezolizumab and bevacizumab in advanced hepatocellular carcinoma (HCC): Indirect treatment comparisons (ITC) using the RESORCE study.

    Author
    Publication venue
    American Society of Clinical Oncology (ASCO)
    Publication date
    Field of study
    Full text link
    537 Background: Our objective was to explore ITC of sequential therapy with sorafenib followed by regorafenib (sorego) against atezolizumab+bevacizumab (atezobev) in HCC. Naïvely comparing median Overall Survival (OS) from sorafenib initiation on sorego in the RESORCE trial with atezobev from IMbrave150 is biased by population imbalance and selection of patients who survived on sorafenib in RESORCE. We used unanchored Matching Adjusted Indirect Comparison (MAIC) and novel survivorship bias adjusted anchored ITC (SBITC) to overcome these limitations. Methods: Overall Survival (OS) from sorafenib initiation in RESORCE on sorego were compared with reconstructed Individual Patient Data (IPD) OS based on Kaplan-Meier curves from IMbrave150 on atezobev. In MAIC, the sorego arm of RESORCE was weighted on reported baseline characteristics to match the IMbrave150 atezobev arm. In SBITC, sorafenib IPD from IMbrave150 were sampled with replacement and added to the RESORCE BSC arm to correct for “survivorship bias”. Sample size was sufficient for follow-up prior to RESORCE randomisation to be within 5% of sorafenib follow-up in IMbrave150. Anchored Bucher ITC was used to compare to unadjusted IMbrave150 with bootstrapping to obtain standard errors. Hazard Ratios (HRs) were used to compare OS. Results: MAIC gave an OS HR of 0.75 (95% confidence interval 0.60, 0.93; p-value=0.007). Effective sample size (ESS) was reduced from 379 to 271.75. SBITC gave a HR of 0.63 (0.52, 0.76; p-value &lt;0.001) requiring an average of 83 sorafenib patients to be added from IMbrave150. Conclusions: We proposed MAIC and SBITC to overcome biases in naïve comparisons of OS on sorego with atezobev in HCC. However, MAIC is limited to baseline characteristics in IMbrave150 and does not adjust for “survivorship bias”. SBITC depends on sample size from IMbrave150, and makes no adjustment for imbalance in baseline characteristics. Despite these limitations, our methods were consistent with naïve comparisons and suggested longer OS with sorego compared to atezobev in patients with HCC. </jats:p
    Crossref

    Style of pictorial representation is shaped by intergroup contact

    Author
    Publication venue
    Cambridge University Press (CUP)
    Publication date
    Field of study
    No full text
    Abstract</jats:p
    Crossref

    Evaluating Student Perceptions of a Course-Embedded Faculty Advising Model

    Author
    Publication venue
    North American Business Press
    Publication date
    Field of study
    No full text
    This study evaluated student perceptions of a course-embedded faculty advising model within an online MA program. Faculty advising contributes to student degree completion and career success. Instructor presence, community, the incorporation of synchronous sessions, and the use of web-conferencing software impact student engagement, which leads to increased student satisfaction and retention. The course-embedded structure of model contributes to reduced resource needs and high levels of sustainability. Structured, individual synchronous faculty advising sessions were built into 4 courses and student experience was assessed utilizing a survey. Results are discussed in the context of relevant theories and best practices
    Article Gateway

    Early Predictors of Near-Shore Spinal Injuries Among Emergency Department Patients

    Author
    Publication venue
    Elsevier BV
    Publication date
    Field of study
    Full text link
    Crossref
    • …
    corecore